Scientific note

Susceptibility of the predator Euborellia annulipes (Dermaptera: Anisolabididae) to mycoinsecticides

Susceptibilidad del depredador Euborellia annulipes (Dermaptera: Anisolabididae) a micoinsecticidas

FLÁVIA QUEIROZ DE OLIVEIRA¹, JACINTO DE LUNA BATISTA¹, JOSÉ BRUNO MALAQUIAS³, CARLOS HENRIQUE DE BRITO⁴, and EMMERSON PEREIRA DOS SANTOS⁵

¹ M.Sc. Student in Environmental Science and Technology, Universidade Estadual da Paraíba (UEPB), Brazil. e-mail: flavinha2010@ibest.com.br corresponding author;

² Ph.D., Universidade Federal da Paraíba (UFPB). Campus II - Areia- PB. e-mail: jacinto@cca.ufpb.br;

³ M.Sc. Student in Entomology, Universidade de São Paulo (USP), Brazil. e-mail: jbmalaquias@ig.com.br;

⁴ Researcher/Professor, Universidade Federal da Paraíba (UFPB), Campus II - Areia- PB. e-mail: chbrito1@hotmail.com;

⁵ B.Sc. Universidade Federal da Paraíba (UFPB), Campus II - Areia - PB. e-mail: emmersonbiozoo@hotmail.com.

Received: 23-dec-2010 - Accepted: 2-jun-2011

Abstract: To evaluate the infuence of Beauveria bassiana (Balsamo) Vuill and Metarhizium anisopliae (Metsch) Sorok on the survival of the predator Euborellia annulipes Lucas (Dermaptera: Anisolabididae) were used product concentrations 5.00x10⁹; 7.50x10⁹; 10.00x10⁹; 12.50x10⁹ conidia/L from isolates of B. bassiana and M. anisopliae which were obtained, respectively, through products Boveril^® and Metarril^® and control (0.0 conidia/L). High rates of hatching nymphs of E. annulipes have been recorded in clutches that received applications of M. anisopliae. Moreover, B. bassiana has affected negatively the hatching rate of nymphs of E. annulipes. A slightly harmful effect has been observed in all concentrations of conidia B. bassiana in clutches of E. annulipes. The survival was 100% in all tested concentrations of M. anisopliae in females of E. annulipes. The females which received topical application of B. bassiana have shown a survival rate ranging from 80.30 (12.50x10⁹ conidia) to 100% (5.00x10⁹ conidia). In males of E. annulipes, the survival rate in insects treated with B. bassiana ranged from 95.00 (12.50x10⁹ conidia) to 100% (5.00x10⁹ conidia), while with M. anisopliae applications this rate was 96.02 (12.50x10⁹ conidia) to 100% (5.00x10⁹ conidia). The entomopathogenic fungi B. bassiana and M. anisopliae did not affect the mortality of nymphs and adults of E. annulipes. However, careful measures should be adopted in applications of B. bassiana directly into clutches of E. annulipes.

Key words: Pathogenicity. Entomopathogenic fungi. Selectivity. Natural enemy.

Resumen: Se evaluó la infuencia de Beauveria bassiana (Balsamo) Vuill y Metarhizium anisopliae (Metsch) Sorok en la supervivencia del depredador Euborellia annulipes Lucas (Dermaptera: Anisolabididae). Las concentraciones de los productos evaluados fueron 5,00x10⁹; 7,50x10⁹; 10,00x10⁹; 12,50x10⁹ conídios/L de aislamientos de B. bassiana y M. anisopliae, respectivamente, con los productos Metarril^® y Boveril^® y un control (0,0 conidios / L). Se registraron altas tasas de eclosión de ninfas de E. annulipes en especímenes que recibieron tratamientos con M. anisopliae. Se observó un efecto levemente perjudicial en todas las concentraciones de conidios de B. bassiana en posturas de E. annulipes, pues B. bassiana afectó negativamente la tasa de eclosión de ninfas de E. annulipes. La supervivencia fue del 100% en hembras de E. annulipes que recibieron todas las concentraciones probadas de M. anisopliae. Las hembras que recibieron aplicaciones tópicas de B. bassiana presentaron sobrevivencia entre 80,3 (12,50x10⁹ conidios) y 100% (5,00x10⁹ conidios). Los machos de E. annulipes presentaron una tasa de supervivencia con B. bassiana entre 95 (12,50x10⁹ conidios) y 100% (5,00x10⁹ conidios) y de 96,0 (12,50x10⁹ conidios) a 100% (5,00x10⁹ conidios) al recibir aplicaciones de M. anisopliae. Los hongos entomopatógenos B. bassiana y M. anisopliae no afectaron la mortalidad de ninfas y adultos de E. annulipes. Sin embargo, se deben adoptar medidas precisas en aplicaciones de B. bassiana directamente en posturas de E. annulipes.

Palabras clave: Patogenicidad de hongos. Hongos entomopatógenos. Seletividad. Enemigo natural.

Introduction

Earwigs or Dermaptera constitute several species with high predation capacity due to their aggresive behavior. Among these species, Euborellia annulipes (Lucas, 1847) (Dermaptera: Anisolabididae) is an important biological control agent of pests in agriculture. This insect species occurs throughout the neotropical zoogeographical region and has been an potential predator of several insect pests, particularly of eggs and immature stages of insects of the Lepidoptera, Hemiptera, Coleoptera and Diptera (Lemos et al. 1998, 1999). In     1998). Innortheast Brazil it is common occurrence of this insect preying on immature stages of pests that occur in soil or in plant  structures lying on the ground as case of the larvae and pupae  of boll weevil at feld of cotton.   

Entomopathogens are prevalent in natural systems and should receive greater attention in life-history studies (Hesketh et al. 2010; Roy et al. 2009). Mycoinsecticides compose only a small component of the biopesticide market, but their use is gaining acceptance as an alternative to the use of traditional contact insecticides for pest control (Ludwig and Oetting 2001). Beauveria bassiana (Balsamo) Vuill and  Metarhizium anisopliae (Metsch) Sorok are among the most studied species of entomopathogenic and are used as biocontrol agents of agricultural pests (Alves 1992; Alves et al.1998).biological control of pests an important pathogen is  that one who affects only the target insect and not their natural enemies (Flexner et al. 1986; Generoso 2002).  

The severity of the effects of microbial insecticides depends on the concentration of spores applied and the stage of

the insect (Ginsberg et al. 2002). The use of low concentrations of alternative insecticides may increase the selectivity and help reduce costs of production (Oliveira 2008). In recent studies conducted by Oliveira et al. (2010), it was observed that low concentrations of mycoinsecticides isolates of B. bassiana and M. anisopliae promoted high efficiency control of larvae and pupae of fruit fies. However the direct effects of entomopathogenic fungi in natural enemies have not been well studied and probably have been overestimated. It is also necessary to develop techniques to express accuracy in doses of microbial insecticides to achieve maximum physiological compatibility. Due to the increased use of biological control in integrated pest management and considering that information regarding the selectivity of entomopathogenic fungi in biological control agents is incipient, the aim of this research is to evaluate the infuence of M. anisopliae and B. bassiana in the survival of the predator E. annulipes.

Material and Methods

Insects. Specimens of the E. annulipes used in this study were obtained from laboratory rearing. The stock colony was maintained in an acclimatized room at 26±1°C, of 70±10% r.h. and 12L:12D photoperiod. The bioassays followed the methodology described by Lemos et al. (1999). This study used eggs, nymphs of 1st instar, (≤ 24h old), 2nd instar, and adult insects of both sexes of E. annulipes. Individuals have been placed in Petri dishes and kept at a constant temperature of 25±1°C, with 70±10% R.H., and a photoperiod of 12:12h (L:D). The insects or clutches has been kept in groups of 5 units per dish. Recently emerged adults (≤ 24h old) have been sexed by the characteristics of external morphology, the number of abdominal segments and the curvature of the forceps (Tomkins and Simmons 1998).

Bioassay. The following concentrations of conidia tested were 5.00x10⁹, 7.50x10⁹; 10.00x10⁹; 12.50x10⁹ conidia/L, and control (0.0 conidia/L). These concentrations are recommended to the pests control, i.e. in applications at soil to control of larvae and pupae of fruit fies (Oliveira 2008). The isolates have been obtained from the products Boveril^® and Metarril^®, produced by the Company Itaforte Bio products.

Patogenicity and virulence of isolates. The viability of isolated evidence of pathogenicity and virulence has been evaluated according to the method described by França et al. (2006). In the viability test has been used two Petri dishes containing PDA (potato dextrose agar) culture media, incubated in climatized chamber (25±2°C, 12h of photophase and relative humidity of 70±10%) for 24 hours, later to perform readings in a light microscope by determining the percentage of germinated conidia and not germinated. For proving the pathogenicity and virulence of the isolates tested in this study, we spraying a suspension of 5.00x10⁹ conidia/L on the 100 larvae 1st-instar of Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) for each species of fungus, because it is a highly susceptible to entomopathogenic stage (Oliveira 2008).

Effects on survival and development stages of E. annulipes. Fungal suspensions of each fungal product concentration have been applied topically with deposition of 5μl from each suspension. The applications have been performed using a manual spray (1L) just once time on the entire structure of the dorsal body of the specimens, and in the case of the clutches around the whole egg chorion. After 10 days of period application was observed the survival rate. This period was established because the action time of two microorganisms have been also observed and the assessments made after 5 days of application to the insects as young and adult (Bustillo et al. 1999) as well as after 10 days, which was the maximum period of hatching in this conditions (Lemos 1998). The mortality was confrmed by sporulation in humide chamber. Then, mortality has been calculated and corrigid to control (untreated) group.

Data analysis. The experimental design was completely randomized with fve replications per concentration/treatment, with each replication consisting of fve insects or clutches (100 eggs per clutch). The survival of insects in treatments was calculated using the formula: S (%) = 100 - Ma (%). The mortality data (Ma %) of the treatments and control were submitted to Abbott’s formula (Abbott 1925). Data were also subjected to polynomial logistic regression (P=0.05) (PROC GENMOD, SAS Institute 2006). The determination of the harmfulness of the products was based on the guidelines for the insecticides under laboratory conditions by the IOBC/ WPRS (Amano and Haseeb 2001): survival >70%, non-harmful, survival >30% and <70%, slightly harmful; survival> 1% and <30%, moderately harmful and survival <1% severely harmful.

Results and Discussion

In our test with C. capitata, it has been recorded a mortality rate approximately 98% and 97% caused by fungi M. aniso-pliae and B. bassiana, respectively. Therefore, the survival of E. annulipes was not because the lack of fungi virulence.

High rates of hatching nymphs of E. annulipes have been recorded in clutches that received applications of M. aniso-pliae. The hatching percentage nymphs confrmed was 96.23, 92.47, 91.58 and 91.50% in the concentrations of 5.00x10⁹, 7.50x10⁹, 10.00x10⁹ and 12.50x10⁹ conidia/L of M. aniso-pliae, respectively. Moreover, B. bassiana affected the hatch-ing rate of nymphs of E. annulipes, the survival ranged from 31.13 (12.50x10⁹) to 60.06% (5.00x10⁹) (Fig. 1). Results obtained by Duso et al. (2008) with the predatory mite Phytoseiulus persimilis (Athias-Henriot, 1957) (Acari: Phytosei-idae) have shown high hatching rate of this predator species after application of B. bassiana, although this researchers have reported mortality of up to 43% in females.

In the other hand, M. anisopliae and B. bassiana did not affect the survival of nymphs. There was overall survival (100%) of nymphs of 1st and 2nd instars in all tested concentrations of both the fungi B. bassiana and M. anisopliae (data not shown in fgures). Results obtained by Kohno et al. (2007) are in agreement with those obtained in this study. These authors verifed no effect of B. bassiana to nymphs of 2nd instar Labidura riparia (Pallas, 1773) (Dermaptera, Labiduridae).

The fungi M. anisopliae and B. bassiana did not affect the survival of adults of E. annulipes. In all tested concentrations of M. anisopliae in female E. annulipes, survival was 100%. The females which received topical application of B. bassiana have been shown a survival rate ranging from 80.3% (12.10x10⁹ conidia) up to 100% (5.00x10⁹ conidia); whereas, for males of E. annulipes, the survival rate in insects treated with B. bassiana ranged from 95% (12.10x10⁹ conidia) to 100% (5.00x10⁹ conidia) and from 96.02% (12.10x10⁹ conidia) to 100% (5.00x10⁹ conidia) after receiving applications of M. anisopliae (Figs. 2a and 2b).

Efficient selectivity is based on physiological differences between pests and their natural enemies, being that pest killed at a concentration of product that does not affect individual benefts. A slightly harmful effect (survival> 30% and <70%) has been observed in all the concentrations of conidia B. bassiana in clutches of E. annulipes (Fig. 1). The microbial insecticides tested were not harmful (survival> 70%) for adults (Figs. 2A and 2B) and nymphs of frst and second instar. Therefore, the two mycoinsecticides can be used to control pests without significantly affecting nymphs or adults of E. annulipes. The greater susceptibility of eggs of E. annulipes in relation to nymphs or adults should be the interaction that occurs due to pathophysiologic effects on the cuticle of nymphs or adults of the predator, which hindered the process of infection and lessens the potential for invasion of microorganisms for these stages of E. annulipes. More-over, the ability of this insect to survive in areas of land, soil inhabitant galleries can to be an important factor to provide a greater hardening of their cuticle. Other factors such as intestinal pH, biochemical or immunological defense-physiological may be related to the tolerance of nymphs or adults of E. annulipes concentrations of mycoinseticidas used in this study.

According Kiselek (1975), B. bassiana was harmless to adults Cryptolaemus montrouzieri Mulsant, 1853 (Coc-cinellidae: Coleoptera) while the effect was lethal to larvae (50%) that consumed insects infected by this fungus. In this study, nymphs and adults of E. annulipes were not affected by concentrations tested in this study adopted of B. bassiana and M. anisopliae. In other words, the results of this study have revealed that B. bassiana caused a reduction in the rates of hatched nymphs, unlike M. anisopliae. More-over, insecticide applications that are not directly to the soil may enable the adoption of that microorganism in programs of integrated pest management in agroecosystems in which E. annulipes is present, because this kind of predator tends to deposit their eggs in soil galleries built by adults (Bha-radwaj 1966).

Other studies have also demonstrated the selectivity of entomopathogenic organisms to natural enemies, like B. bassiana with Orius insidiosus (Say, 1832) (Hemiptera: An-thocoridae) (Cavalcanti 2006); M. anisopliae and B. bassiana with parasitoid Oomyzus sokolowskii (Kurdjumov, 1912) (Hymenoptera: Eulophidae) (Santos Jr. et al. 2006). The con-centrations adopted in this study of entomopathogenic fungi B. bassiana and M. anisopliae did not affect the mortality of nymphs and adults of E. annulipes. Neither we found ovicid-al activity of M. anisopliae to E. annulipes. However, care-ful measures should be adopted in applications from 5.0x10⁹ conidia/L of B. bassiana directly into clutches of E. annuli-pes, because from this concentration we found a significant reduction in hatching rate this predator. We also suggest the implementation of other studies, as the effects these bio-insecticides on the ftness of E. annulipes.

Acknowledgements

The authors thank the Brazilian agency Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) to concession scholarship to frst author. The authors would like to thank Robério de Oliveira for his help in the laboratory.

We especially thank Tricia Hornsby (Ohio State University) for her valuable help with an important review of English in this manuscript.

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