Abstract

BLANCO FLORES, Héctor Alexander; VARGAS CEDENO, Teresa Edith  and  GARCIA DE GARCIA, Eva Cristina. In vitro regeneration of Amazonian pineapple ( Ananas comosus ) plants ecotype Gobernadora. Rev. colomb. biotecnol [online]. 2017, vol.19, n.1, pp.7-20. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v19n1.65561.

There are a number of pineapple (Ananas comosus) cultivars and ecotypes of local commercial importance in Venezuela, among them the Amazonian ones, cultivated mainly by the aboriginal Piaroa, are of relevance. They sow the propagules, which restricts the availability of material for large-scale cultivation. This limitation was approached by plant tissue culture for in vitro propagation of Amazonian pineapple plants, Gobernadora ecotype, through somatic embryogenesis (ES) and adventitious organogenesis (OA). Basal and intermediate sections of leaves were tested. Only the leaf base sections (FBS) were morphogenically induced. The highest number of vitroplants (1.58 plants / explant) was obtained from the embryogenic callus induced in MS medium with Picloram 10 mg.L-1 + Thidiazuron 2 mg.L-1, transferred to MS medium without hormones. In the organogenic process, the highest number of plants / explants (5) was obtained directly in MS with naphthaleneacetic acid 5 mg.L-1 + benzylaminopurine 0.25 mg.L-1, transferred to MS. The latter being the best in vitro culture system due to its productivity and for being a method that minimizes somaclonal variation.

Keywords : Leaf base; somatic embryogenesis; adventitious organogenesis; naphthaleneacetic acid; Thidiazuron.

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