Abstract

RAMIREZ, Edilene  and  MARTINEZ, Sixta T. Determination of extra and intracellular lytic enzymes related with carnation (Dianthuscaryophyllus L.) root cell wall. Rev.Colomb.Quim. [online]. 2016, vol.45, n.2, pp.5-11. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v45n2.60370.

The presence of some enzymes related to cell wall (polygalacturonase, the pectate lyase, protease and xylanase) in carnation (Dianthus caryophyllus L.) roots as well as the activity levels were determined. These levels were analyzed in different cellular places: the intercellular fluid that is part of the apoplast, the symplast, and the total level (apoplast and symplast) in carnation roots. Two methods were tested to extract the intercellular fluid. To obtain the intracellular content (symplast) and total extract (apoplast+symplast), three methods were tested, using as extracting solution i) phosphate buffer, ii) phosphate buffer + PVPP, iii) before the extraction with phosphate buffer, the carnation roots were washed with acetone. The results showed the effect of different extracting solutions in the enzymatic activities and in the protein content. A new only one step method is proposed to extract the four enzymes and make the comparative analysis of enzymatic activity.

Keywords : Intercellular fluid; intracellular extract; enzymatic activity; polygalacturonase; pectate lyase; protease; xylanase.

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