Abstract

AGAMEZ RAMOS, ELKIN; BARRERA VIOLETH, JOSé  and  OVIEDO ZUMAQUE, LUIS. Evaluation Of Antagonism And Multiplication The Trichodermasp. In The Middle Static Liquid Banana Substrate. Acta biol.Colomb. [online]. 2009, vol.14, n.3, pp.61-70. ISSN 0120-548X.

The antagonistisc ability of nine isolated natives fungi Trichoderma sp from the collection of strains at Laboratory of phytopathology at University of Cordoba, Colombia was assessed so as its production of spores in crop liquid static medium, cross the in vitro proof antagonism using two phytopathogenic fungi: Fusarium sp. and Rhizoctonia sp., this proof let select the isolated with the major antagonistic activity and adjust the production of spores through fermentation process in liquid static by means of buffer and without buffer with phosphate 0,2 N, being used as principal substrate plantain flour (HP) 5%, besides of nutritional supplements that formed the trataments: HPM (molasses solution 5%), HPL (granulated yeast solution at 2%), HPU (urea solution 0.1%), and as witness PGL (potato glucose liquid). In the test of antagonism was selected the isolated C2 of Trichoderma sp. by present the major antagonistic ability in front of the fungi Fusarium sp., with a inhibition percentage at 78,30% and reporting a index 4 of antagonism, in the process of spores production, the tratament HPL (plantain flour plus yeast without buffer) stressed for the favorable production of conidias, with a concentration of 1,1 x 10⁹ conidias/mL. The buffer process were significant differentiated, showing that the variable buffer influenced in the production of conidias, the mediums that shocked in the production show 1,01 x 10⁹ conidias/mL, Ïwith respect at don’t shocked 6,3 x 10⁸ conidias/mL.

Keywords : Trichoderma sp; Fusarium sp; Rhizoctonia sp; antagonism; spores; liquid fermentation.

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