Abstract

VENTURA, CESAR E; RAMIREZ, GLORIA  and  VERA, VÍCTOR. Detection and Differentiation of Mycoplasma gallisepticum and Mycoplasma synoviaeby PCR from Tracheal Swabs from Birds with Respiratory Symptoms. Acta biol.Colomb. [online]. 2012, vol.17, n.3, pp.525-536. ISSN 0120-548X.

Mycoplasmas are worldwide pathogens that affect the poultry industry causing respiratory illness which cause a negative economic impact. Two mycoplasmas species are the most important in the commercial poultry: Mycoplasma gallisepticum(MG) and Mycoplasma synoviae(MS). By its importance and necessity to know and differentiate between mycoplasmas species in locals poultry houses this study used the PCR technique like a diagnosis tool, using tracheal swabs from bird with respiratory symptoms. A total of 91 samples from broilers, layers and breeders farms located in the departments of Cundinamarca and Boyacá was processed. The punctual prevalence founded in this study was 39.6 % for MG and 47.3 % for MS. Statistical differences for type of production and positive samples for MG y MS (p < 0.05) were founded, a bigger number of positive samples from layers and breeder in comparison to broilers was found. In the same way, the positive samples for the layers and breeder from the age group between 20 and 60 weeks was greater, while for the broilers group most of the positive samples were from five weeks old birds for MG and two weeks old birds for MS.

Keywords : Mycoplasma gallisepticum; Mycoplasma synoviae; PCR.

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