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Boletín Científico. Centro de Museos. Museo de Historia Natural
Print version ISSN 0123-3068
Abstract
RESTREPO-GARCIA, Ana María; BERNAL-ARIAS, Laura and SOTO-GIRALDO, Alberto. Eurhizococcus colombianus Jakubski (1965) (Hemiptera: Margarodidae): Evaluation of its biological control using a mixture of the entomopathogenic nematodes Steinernema (Rhabditida: Steinernematidae) and Heterorhabditis (Rhabditida: Heterorhabditidae). Bol. Cient. Mus. Hist. Nat. Univ. Caldas [online]. 2022, vol.26, n.1, pp.155-168. Epub June 14, 2022. ISSN 0123-3068. https://doi.org/10.17151/bccm.2022.26.1.11.
Objectives:
To isolate native entomopathogenic nematodes (NEP) and evaluate the pathogenicity of the mixture of Steinernema sp. and Heterorhabditis sp. on Eurhizococcus colombianus under laboratory conditions. Scope: The mixture of native entomopathogenic nematodes of the Steinernema and Heterorhabditis genera efficiently control E. colombianus.
Methodology:
Native NEPs were isolated using Galleria mellonella larvae as a trap insect, both nematodes were mixed and their pathogenicity was evaluated on the development stages of E. colombianus.
Results:
Native NEPs of the Steinernema and Heterorhabditis genera were isolated and mixed. Twenty-four h after the E. colombianus infestation on females, mortality of 95% was presented at the dose of 100 JI / 25μl, while at 192 h mortality was 100% in the doses of 10 and 100 JI / 25μl. When NEPs were applied to E. colombianus cysts in doses of 10, 100 and 1000 JI / 25 μl, their rupture was 43%, 21% and 24%, respectively.
Conclusions:
The mixture of the isolated NEPs efficiently control the development stages of E. colombianus at the doses evaluated.
Keywords : Ground pearl; biological control; integrated pest management.