Services on Demand
Journal
Article
Indicators
- Cited by SciELO
- Access statistics
Related links
- Cited by Google
- Similars in SciELO
- Similars in Google
Share
Acta Biológica Colombiana
Print version ISSN 0120-548X
Abstract
RODRIGUEZ CASTRO, JOHANNA PAOLA; NARVAEZ CUENCA, CARLOS EDUARDO and RESTREPO SANCHEZ, LUZ PATRICIA. Polygalacturonase Activity in Yellow Pitaya Peel (Acanthocereus pitajaya). Acta biol.Colomb. [online]. 2006, vol.11, suppl.1, pp.65-74. ISSN 0120-548X.
Polygalacturonase activity was determinated in yellow pitaya fruit (Acanthocereus pitajaya). Protein concentrations and activities of the enzyme in the extracts were determined. Buffer sodium phosphate 20 mM pH 7,0 with NaCl 0,5 M was the most effective extraction system. The enzyme exhibited optimum activity at pH 5,0 in buffer citrate at 40 °C. The KM of the enzyme was 2.9 mg/mL for polygalacturonic acid and VMÁX of 0.076 nmol of reducing groups/s. Results show that polygalacturonase is related with softening on yellow pitaya fruit.
Keywords : Polygalacturonase; yellow pitaya; enzyme of softening.