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Acta Biológica Colombiana
Print version ISSN 0120-548X
Abstract
MARTINEZ ABAD, Lily Paola et al. A NEW RECORD OF Leishmania (Viannia) guyanensis (Trypanosomatidae) FROM THE PACIFIC COAST OF COLOMBIA. Acta biol.Colomb. [online]. 2021, vol.26, n.1, pp.135-138. Epub Mar 18, 2021. ISSN 0120-548X. https://doi.org/10.15446/abc.v26n1.78321.
The aim of this study was the identification of Leishmania species that causes cutaneous leishmaniasis in a patient from Buenaventura, Valle del Cauca, on the Pacific coast of Colombia. Clinical samples were obtained from a 29 years-old male who presented a distinct ulcer with raised borders on his neck. Samples were taken for direct microscopic examination, parasite culture, and molecular identification of the infecting Leishmania species by sequencing of the cytochrome b gene. Direct examination was positive for amastigotes of Leishmania but the culture was negative. The infecting parasite species was identified as L. (V.) guyanensis by means of the nucleotide sequence of a 509 bp fragment of the cytochrome b gene. We report the presence of L. (V.) guyanensis in rural areas of Buenaventura in Valle del Cauca, and the expansion of the geographical distribution of this species in the Pacific region of Colombia.
Keywords : cutaneous leishmaniases; cytochrome b; epidemiology; parasites.