Abstract

MURILLO, Elizabeth; TIQUE, Margarita María; OSPINA, Luis Fernando  and  LOMBO, Óscar. Preliminary evaluation of hypoglycemic activity in diabetic mice and in vitro antioxidant activity of Bauhinia kalbreyeri Harms extracts. Rev. colomb. cienc. quim. farm. [online]. 2006, vol.35, n.1, pp.64-80. ISSN 0034-7418.

Bauhinia kalbreyeri (“cow hof ”), is a tropical plant included in the family Caesalpinaceae. In this work, an ethanolic was obtained. From this extract two bioassays were carried out in order to determine the hypoglycemic action of plant. The study was complemented evaluating the antioxidant activity by the DPPH free radical method and carrying out a preliminary phytochemical analysis. The diabetes was induced in normoglycemic mice by means of aloxane administration in dose of 75 mg/Kg. The diabetic animals were assigned to different groups to be subjected to treatment with the extract (1.000 mg/Kg), the vehicle (control), the tolbutamide (pattern, per os) and the insulin (via subcutaneous). In those bioassays was carried out comparisons by means of a variance analysis (ANOVA). The unique dose of 1.000 mg/Kg did not present antidiabetic activity in mice with diabetes type I (insulin dependent). Nevertheless, the ethanolic and aqueous extracts of leaf and bark showed free radical scavenging capacity comparable to ascorbic acid, which was used as pattern. The antioxidant activity of extracts could be related with the ethnopharmacology use of Bauhinia kalbreyeri as antidiabetic, and being consequence of the abundant presence of phenolic-like compounds detected.

Keywords : Bauhinia kalbreyeri; diabetes; hypoglycemic activity; antioxidant activity; free radical DPPH.

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