Abstract

CARDENAS, Diana Paola et al. Evaluating Clostridium sp. native strains1,3-propanediol production byfermentation from glycerol USP and raw glycerol from biodiesel production. Rev. colomb. cienc. quim. farm. [online]. 2006, vol.35, n.1, pp.120-137. ISSN 0034-7418.

This work was aimed at evaluating 1,3 Propanediol (1,3-PD) production from glycerol in thirteen Clostridium sp. strains isolated from Colombian soils. Two culture media containing USP glycerol (G1A and G1B) and variable concentration of PABA, biotin and mineral stock, and a third medium containing raw glycerol from biodiesel production were evaluated. Volumetric productivity for 1,3-PD was determined in native strains in three media. In average native strains showed 1,6 greater volumetric productivity and a yield 128% higher when grown in G1B medium in comparison to those obtained in G1A medium. Native IBUN13A strain had 50% greater volumetric productivity (Q_{1,3 PD}= 0,060 mg mL^-1 h^-1) than the Clostridium butyricum DSM523 reference strain (Q_{1,3 PD}= 0.030 mg mL^-1 h^-1) and the same parameter was increased by 28% in raw-glycerol medium (G2) regarding that obtained in G1 medium. These results suggest that raw glycerol obtained from the biodiesel process without any purification may be used for producing 1,3-PD at industrial level by optimising fermentation conditions. Transforming this waste into an added-value product should become profitable the biodiesel production process in Colombia.

Keywords : 1,3-PD; Clostridium; glycerol; volumetric productivity; biodiesel.

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