Services on Demand
Journal
Article
Indicators
Cited by SciELO
Access statistics
Related links
Cited by Google
Similars in SciELO
Similars in Google
Share
Revista de la Facultad de Medicina
Print version ISSN 0120-0011
Abstract
PENATA, Adrián et al. Distribution and frequency of potential mutations associated with rifampicin resistance in the rpoB gene of Mycobacterium tuberculosis detected using a molecular automated method. rev.fac.med. [online]. 2023, vol.71, n.1, e5. Epub June 24, 2024. ISSN 0120-0011. https://doi.org/10.15446/revfacmed.v71n1.98079.
Introduction:
Tuberculosis continues to be a public health problem that is now aggravated by drug-resistant Mycobacterium tuberculosis. More than 95% of rifampicin (RIF)-resistant strains of M. tuberculosis have mutations in a region of the rpoB gene. Xpert™ MTB/RIF is a molecular biology system that allows identifying mutations in the RIF-resistance determining region of this gen using 5 probes (A, B, C, D, E) that make up the rpoB gene sequences.
Objective:
To describe the distribution and frequency of potential mutations associated with RIF resistance in the rpoB gene of M. tuberculosis detected in pulmonary and extrapulmonary samples using the Xpert® MTB/RIF method.
Materials and methods:
Retrospective study in which 66 samples positive for RIF-resistant M. tuberculosis, processed using the GeneXpert MTB/RIF system between January 2011 and July 2019 at a university hospital in Medellín, Colombia, were analyzed. According to the Dx System software of the GenXpert instrument, a potential mutation and RIF resistance were established if the probes did not bind to their natural complementary beacon or if there was a delay in binding (delta CT>4) in relation the other probes due to the presence of an abnormal beacon. Data were analyzed using descriptive statistics.
Results:
Of the 66 samples (48 pulmonary and 18 extrapulmonary), 63.64% were from men and participants' mean age was 39.60 ± 17.69 years. The frequency and distribution of mutations was as follows: probe E: 38 mutations (57.58%), B: 16 mutations (24.24%), D: 8 mutations (12.12%), A: 3 mutations (4.54%) and D&E: 1 mutation (1.52%). No mutation was detected in probe C.
Conclusions:
Mutations associated with RIF resistance in the rpoB gene of M. tuberculosis detected by the Xpert® MTB/RIF method were mainly found in probe E (codons 529-533). On the other hand, no mutation was detected in probe C.
Keywords : Colombia; Mutation; Polymerase Chain Reaction; Tuberculosis; Rifampicin (MeSH).