Abstract

CASTRO-VARGAS, Henry I.  and  PARADA-ALFONSO, Fabián. Evaluation of protective effect against lipid oxidation of fractions obtained from tree tomato (Solanum betaceum Sendtn) epicarp. Rev.Colomb.Quim. [online]. 2017, vol.46, n.2, pp.17-23. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v46n2.62988.

Fractions from tree tomato (Solanum betaceum Sendtn) epicarp were obtained and their effect against lipid oxidation in cooked beef meat (CBM) was evaluated. An extract obtained by supercritical fluids extraction was fractionated using solid phase extraction and these fractions were added to CBM (at 300 mg/kg). The protector effect against lipid oxidation was determined by measuring some lipid oxidation products and comparison against the synthetic antioxidant tert-butylhydroquinone (TBHQ at 300 mg/ kg). Four fractions were obtained, the most polar fraction showed high efficiency to reduce the lipid oxidation on CBM, this fraction reduced the LHP and TBARS formation at 100.0 and 98.5%, respectively. This fraction showed an ability to retard lipid oxidation in CRC higher than TBHQ. The fraction with the highest protector effect was analyzed for its phenolic composition by High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD). Three catechin (epigallocatechin, epicatechin and epigallocatechin gallate) and a phenolic acid (rosmarinic acid) were identified. The results revealed that the tree tomato epicarp is a source of antioxidants with protective effect on CBM, an alternative for the use and valorization of this agroindustrial waste.

Keywords : Tree tomato epicarp; Solanum betaceum Sendtn; lipid oxidation; antioxidant activity; supecritical fluids extraction.

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