Abstract

MONROY-MENA, Santiago et al. Selection of reference genes for transcriptional analysis in the pathosystem carnation (Dianthus Caryophyllus L.) - Fusarium oxysporum f. sp. dianthi. Rev.Colomb.Quim. [online]. 2019, vol.48, n.2, pp.5-14.  Epub July 03, 2019. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v48n2.72771.

In this research, reference genes were validated for transcriptional studies in the pathosystem carnation - Fusarium oxysporum f. sp. dianthi. Genes associated with basic cellular processes that have been used as reference genes in other plant-pathogen models were selected and the effect of the inoculation with the pathogen on their expression was determined. For this, it was necessary to design primers for each candidate genes to check their presence in the genome of carnations grown in Colombia and their constitutive transcription in different tissues of the plant using reverse transcription subsequent polymerase chain reaction (RT-PCR). The transcriptional levels of the candidate genes were evaluated using RT-qPCR in stems and roots inoculated with this pathogen for two cultivars with different levels of resistance to the disease. According to the statistical validation carried out using ANOVA and the GeNorm and Normfinder programs, the genes coding for a histone H3 and rRNA18S did not show any significant variation in their expression levels due to inoculation, so they can be used as reference genes in transcriptional studies in this plant-pathogen interaction.

Keywords : carnation; Fusarium oxysporum f. sp. dianthi; RT-qPCR; rRNA 18S; histone.

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