Abstract

JURADO-GAMEZ, H. A; ROMERO-BENAVIDES, D. A  and  MORILLO-GARCES, J. A. INHIBITION OF Lactobacillus gasseri ON Yersinia pseudotuberculosis UNDER IN VITRO CONDITIONS. Rev. Med. Vet. Zoot. [online]. 2016, vol.63, n.2, pp.95-112. ISSN 0120-2952.  https://doi.org/10.15446/rfmvz.v63n2.59357.

Fermentation kinetics, growth tests and inhibition effect of Lactobacillus gasseri on Yersinia pseudotuberculosis was evaluated. Susceptibility to gentamicin, penicillin, ciprofloxacin, and cephalothin, dicloxacillin antibiotics, and the inhibition effect of L. gasseri and supernatant on Y. pseudotuberculosis was determined. Additionally, the growth of L. gasseri to different concentrations of bile salts, bovine bile and temperature. Growth kinetics of lactic strain (CFU/ml, pH, sugar, acidity and protein) was evaluated and peptide in the supernatant was determined by HPLC. The pH and CFU/ml variables were evaluated using a repeated measures time design. L. gasseri and Y. pseudotuberculosis resistance to dicloxacillin was found in addition to the first cephalothin. Lactic strain and its supernatant inhibited the pathogenic strain, 3 x 10⁸ at 1.7 x 1013 CFU/150 pl in bile salts, 3 x 10⁸ at 3 x 10¹² CFU/150 pl in bovine bile and 4.3 x 10⁸ to 3 x 10¹³ CFU/150 pl to different temperatures. Exponential phase was observed at 16 h with a value of 3 x 10¹² CFU/150 pl , pH 4.56, sugar 1.79 mg/l, acidity 0.925 and protein 0.16 mg/l, the TIR-VAL VAL-peptide was found in the supernatant of L. gasseri. The results indicate that L. gasseri can be important as probiotic strain in vitro conditions.

Keywords : Bacterial growth; probiotic; pathogen; antibiotics.

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