Abstract

JURADO-GAMEZ, H; FAJARDO-ARGOTI, I  and  RODRIGUEZ-CAICEDO, A. IN VITRO evaluation OF Lactobacillus gasseri WITH PROBIOTIC CHARACTERISTICS ON Staphylococcus aureus. Rev. Med. Vet. Zoot. [online]. 2016, vol.63, n.3, pp.179-187. ISSN 0120-2952.  https://doi.org/10.15446/rfmvz.v63n3.62745.

Introduction. Lactobacillus gasseri is a lactic bacteria of the digestive tract which hasprobiotic properties important for the control of pathogenic organisms, and Staphylococcusaureus is an important pathogenic strain in the food industry, due to the healthproblems in several countries. Objective. Evaluate the features probiotic of L. gasseri onS. aureus in conditions in vitro. Materials and methods. We evaluated the susceptibilityof two different antibiotic resistant strains; the effect of inhibition of L. gasseri and itssupernatant on S. aureus; growth of the strain lactic to different pH, temperatures, salts bile and bile bovine; the kinetics of fermentation was also established and she foundcount of viable microorganism in plate, pH, sugar consumption, consumption of proteinand percentage of lactic acid; and using HPLC-DAD determined peptides and lacticacid, and in the case of amino acids in the supernatant was determined by HPLC-PDA. Results. Found both strains resistant to antibiotics penicillin, dicloxacillin, cifoxitin andcefalexin. The strain of lactic and supernatant inhibited the growth of S. aureus. Thegrowth was suitable for the different variables with values between 1.5 x 108 to 3.0 x1014 UFC / 150 μl. The exponential phase was observed at 9:00 with values of 33.33Ln UFC/150 μl. Finally were identified using HPLC-DAD VA L-TIR-VA L peptide,11.70 g/l of lactic acid and the amino acid tyrosine. Conclusion. The results show thatL. gasseri has probiotic properties over S. aureus in conditions in vitro.

Keywords : Lactic bacteria; inhibition; pathogenic; probiotic strain.

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