Abstract

PAREJA, ANDREA; MONTOYA, OLGA INÉS  and  GUTIERREZ, PABLO A. Quantitative Protease Assay Using Photographic Films. Acta biol.Colomb. [online]. 2009, vol.14, n.3, pp.217-222. ISSN 0120-548X.

Two quantitative protease assays have been developed based on the classic photographic film test. A discontinuous assay can be performed by measuring the amount of pigment remaining in the film with any image editor software. A continuous assay can be implemented by the measuring the release of silver halide salts bonded in the film using a recording spectrophometer equipped with a Peltier Cell.

Keywords : Protease; Enzyme; Activity assay.

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