Services on Demand
Journal
Article
Indicators
- Cited by SciELO
- Access statistics
Related links
- Cited by Google
- Similars in SciELO
- Similars in Google
Share
Agronomía Colombiana
Print version ISSN 0120-9965
Abstract
RIVERA-TORO, Diana Marcela; VACA-VACA, Juan Carlos and LOPEZ-LOPEZ, Karina. Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops. Agron. colomb. [online]. 2020, vol.38, n.2, pp.218-225. Epub Apr 26, 2021. ISSN 0120-9965. https://doi.org/10.15446/agron.colomb.v38n2.82975.
The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT-PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphisgossypii. This study reports, for the first time, the molecular characterization of CMV in three chili pepper varieties.
Keywords : Cucumovirus; diagnostics; habanero; PCR; Solanaceae; tabasco.