Abstract

MARTINEZ MORENO, Jahnnyer Anilio; HERNANDEZ LOPEZ, Juan Carlos  and  URCUQUI-INCHIMA, Silvio. Stimulation of TLRs, Nod-like receptors and Dectin-1 in neutrophils induces the production of proinflammatory cytokines. Iatreia [online]. 2014, vol.27, n.2, pp.135-146. ISSN 0121-0793.

Objective: To evaluate the expression and function of pattern recognition receptors such as Toll-like receptors, RIG-I/MDA5, NOD-like receptors, Dectin-1 and adaptor proteins, in human neutrophils. Methods: Neutrophils from peripheral blood of healthy individuals were purified and cultured in RPMI-1640, in the presence or absence of specific agonists of the receptor of interest. The expression of pattern recognition receptors was determined by RT-PCR and the secretion of proinflammatory cytokines, by ELISA. Results: We observed that neutrophils express diverse patterns recognition receptors and adaptor molecules. Stimulation of TLR4, TLR5 and TLR7/8 induces the production of IL-1β and IL-6, and activation of Dectin-1 leads to secretion of high levels of TNF-α, but low levels of IL-1β and IL-6. Conclusion: Neutrophils express a large number of pattern recognition receptors and their activation leads to the expression of proinflammatory cytokines.

Keywords : C-type Lectin Receptors; Neutrophils; NOD-Like Receptors; Pattern Recognition Receptors; Pro-Inflammatory Cytokines; RIG-I-Like Receptors; Toll-Like Receptors.

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