Abstract

SANCHEZ RENGIFO, Paula Andrea; PIRAGAUTA AGUILAR (Q.E.P.D.), Miguel Ángel  and  RIANO MELO, Orlando. Biodegradation of polyethylegeglycol 400 with consortium of Pseudomonas sp. and Rhizobium trifolii. Rev. colomb. biotecnol [online]. 2023, vol.25, n.1, pp.69-77.  Epub Dec 11, 2023. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v25n1.91128.

The biodegradation of polyethylene glycol of molecular weight 400 - PEG 400 was evaluated with the consortium Pseudomonas sp. and Rhizobium trifolii in Batch reactors. To determine the optimal operating conditions of the reactor, a medium with minimum essential salts supplemented with CSMEP peptone was prepared; In these media, the behavior of the consortium was estimated with temperature conditions between 15-37°C, pH in a range of 5-9 and PEG 400 concentration of .1-2%. With the results obtained in these previous tests, three Batch - BK reactors were assembled. 14 samples of the contents of each one of the reactors were analyzed during 20 days to measure the biodegradation of PEG 400 using chemical oxygen demand - COD in a closed digestion system. A decrease of up to 98.5% of the polyether was obtained, which showed that the symbiosis was effective to achieve an important removal of the contaminant.

Keywords : consortium; Batch reactor; kinetics; biodegradation.

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