Abstract

GIL, LINA MARCELA; MANJARRES-PINZON, KATHERINE; PINEROS-CASTRO, YINETH  and  RODRIGUEZ-SANDOVAL, EDUARDO. INFLUENCE THE ADDITION OF NITROGEN SOURCE ON PRODUCTION OF LIGNINASES. Rev.Bio.Agro [online]. 2012, vol.10, n.1, pp.173-181. ISSN 1692-3561.

Large amounts of waste rich in lignin and hemicelullose named empty fruit brunch or “raquis” are produced during palm oil extraction process in Colombia. These waste products were a good substrate for growing of Pleurotus ostreatus in solid phase during 10 days of fermentation at 28°C. The aim of this study was to evaluate the enzymatic activity of laccase, manganese peroxidase and lignin peroxidase from the culture of Pleurotus ostreatus on the empty fruit brunch of palm oil with different particle size (2, 3 and 4 cm) and nitrogen source (NH₄Cl and KNO₃). The activity of lignin peroxidase was not detected in the substrate without supplement. On the other hand, the better conditions for laccase activity (7,42 U/L) was in the substrate without supplement and with particle size of 3 cm, and for manganese peroxidase (20,29 U/L) was in the substrate supplemented with ammonium chloride (NH₄Cl) in carbon/nitrogen ratio of 20 and with particle size of 3 cm.

Keywords : Pleurotus ostreatus; Lignin; Lacasse; Palm oil wastes.

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