Services on Demand
Journal
Article
Indicators
Cited by SciELO
Access statistics
Related links
Cited by Google
Similars in SciELO
Similars in Google
Share
Revista Ciencias de la Salud
Print version ISSN 1692-7273On-line version ISSN 2145-4507
Abstract
CHUAIRE-NOACK, Lilian; MONTERREY-GUTIERREZ, Pedro and PAYAN-GOMEZ, César E.. Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells. Rev. Cienc. Salud [online]. 2011, vol.9, n.3, pp.219-228. ISSN 1692-7273.
GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro.
Keywords : gene expression; cell aging; replicative senescence.