Abstract

ARIZA, Yesid  and  SANCHEZ, Ligia. Determination of secondary metabolites from Bacillus subtilis with effect biological control on Fusarium sp.. Nova [online]. 2012, vol.10, n.18, pp.149-155. ISSN 1794-2470.

The use of microorganisms for biological control of plants diseases in an effective alternative that reduce pesticides use that affect soil fertility. Bacillus genus has become a point of interest due the production of secondary metabolites with antifungal properties effective against several phytopathogenics such as Fusarium, Pythium, Phytophthora and Rhizoctonia. The aim of this study was to identify the presence of secondary metabolites produced by liquid fermentation from Bacillus subtilis, analyzed by High-performance liquid chromatography (HPLC), and confirm its biocontrol effect on Fusarium sp. The antibiotic iturin A was identified, with a concentration around 151,805 mg/l. In vitro antagonism tests showed 70 to 100% growth inhibition of Fusarium sp. In the near future is expected to identify the existence of other secondary metabolites with possible significant effect on the plant protection, such as surfactin and fengycin.

Keywords : B. subtilis; Biocontrol; Fusarium sp; High-performance liquid chromatography (HPLC); Iturina A.

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