Abstract

MANRIQUE, Edwin; RINCON, Verónica  and  OSSA, Humberto. Lymphotherapy induce an increase of blocking factors and correct infertility problems. Nova [online]. 2013, vol.11, n.19, pp.25-31. ISSN 1794-2470.

This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique. It was demonstrated BFs presence in MLC in female rabbit groups. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.

Keywords : lymphotherapy; blocking factors; mixed lymphocytes culture; subfertiles females rabbit and fetal allograft.

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