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Entre Ciencia e Ingeniería
Print version ISSN 1909-8367
Abstract
SARRIA-VILLA, R. A.; GALLO, J. A. and BENITEZ, R.. Extraction of phenolic compounds and content of catechin in barks of three forest species from Cauca-Colombia. Entre Ciencia e Ingenieria [online]. 2021, vol.15, n.29, pp.19-27. Epub Feb 20, 2022. ISSN 1909-8367. https://doi.org/10.31908/19098367.2622.
The catechin content in barks of the forest species Pinus patula, Pinus oocarpa and Eucaliptus grandis, cultivated in the Department of Cauca-Colombia, was evaluated. The ethanol extracts were obtained by evaluating the best conditions of particle size, agitation time, solvent-bark ratio and temperature. The catechin content was determined using high performance liquid chromatography (HPLC) with ultraviolet detector and using solid phase extraction (SPE) for sample cleaning. A catechin content of 2.00 ± 0.03%, 2.96 ± 0.02% and 0.18 ± 0.03% was found in barks of P. patula, P. oocarpa and E. grandis respectively. Catechin content indicates the great potential in industrial and medicinal applications for a renewable resource such as bark, abundant in the region and whose field disposition environmentally affects forest areas.
Keywords : Biomass; HPLC; SPE; polyphenols; condensed tannins.