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Biomédica
Print version ISSN 0120-4157
Abstract
MURUA-LOPEZ, Carolina del Carmen; GONZALEZ-OROZCO, María and LOPEZ-MORENO, Héctor Samuel. The plasmacytoid dendritic cells evoke Salmonella-specific CTL effector response. Biomédica [online]. 2019, vol.39, suppl.2, pp.172-181. ISSN 0120-4157. https://doi.org/10.7705/biomedica.v39i3.4349.
Introduction:
The immunological role of plasmacytoid dendritic cells (pDC) in bacterial infections such as Salmonella has been poorly documented. Therefore, we analyzed the effector function of these cells by presenting cytotoxic T lymphocytes (CTL) with Salmonella Typhimurium antigens.
Objective:
To analyze the Salmonella-specific CTL response evoked by pDCs.
Materials and methods:
We used plasmacytoid dendritic cells stained with carboxyfluorescein succinimidyl ester (CFSE) and pulsed with OmpC73, Salmonella Kb- restricted epitopes or S. Typhimurium as targets for cytotoxicity assays.
Results:
Specific lysis was shown to be statistically significant in pDC + OmpC73 for all effector:target ratios (p≤0.05). For pDC + S. Typhimurium, statistical significance was only observed at a 1:100 ratio (p≤0.05) using OmpC73.
Conclusion:
Plasmacytoid dendritic cells evoke CTL response during S. Typhimurium infection.
Keywords : Dendritic cells; T-lymphocytes, cytotoxic; Salmonella; cytotoxicity, immunologic; epitopes.
