Abstract

RESTREPO, Giovanni; OCAMPO, Daniel  and  VELASQUEZ, Andrés. ASSESSMENT OF CRYOPRESERVED SPERM MOTILITY FROM COLOMBIAN CREOLE STALLIONS BY SPERM CLASS ANALYZER. rev.udcaactual.divulg.cient. [online]. 2013, vol.16, n.2, pp.445-450. ISSN 0123-4226.

Reproduction plays a key role in the genetic improvement of horses. Equine semen cryopreservation is a complementary technique to reproductive biotechnology processes. However, only 40% of the horses produce semen with a high variability in fertility that can be cryopreserved. The reliability of methods of semen evaluation is important to determine their fertility potential, however the conventional analysis by microscopic evaluation has not been very effective in this regard. Computer-assisted semen analysis (CASA) provides an objective measurement of sperm motility. This study aimed to compare the evaluation of equine sperm motility through conventional and SCA® (CASA) methods. Semen of three horses of the Colombian creole breed (three ejaculates each) was frozen in extender (4% egg yolk, 5% dimethylformamide). Thawed sperm motility was conventionally determined by phase contrast microscopy and by SCA ® software. For statistical analysis a generalized linear model (GLM) was fitted and the association between variables, using correlation and regression analysis, was determined. An average of total motility of 54.3% ± 10.1% and 61.8% ± 13.9% for conventional and SCA® evaluation, respectively, was detected.. No significant difference was observed between the methods of analysis. It is concluded that equine semen analysis by conventional method and SCA® system is comparable for analyzing the motility of criopreserved equine semen.

Keywords : Biotechnology; fertility; sperm motility; CASA.

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