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Acta Biológica Colombiana
Print version ISSN 0120-548X
Abstract
HUESPE, Ivan et al. Liver Melanomacrophages and Gluthation S-Transferase Activity in Leptodactylus chaquensis (Anura, Leptodactylidae) as Biomarkers of Oxidative Stress Due to Chlorpyrifos Exposition. Acta biol.Colomb. [online]. 2017, vol.22, n.2, pp.234-237. ISSN 0120-548X. https://doi.org/10.15446/abc.v22n2.60823.
ABSTRACT We quantified and compared the hepatic melanomacrophage (MM) and glutathione S-transferase (GST) enzyme activity (two oxidative stress biomarkers) in the liver of Leptodatylus chaquensis adults (Anura, Leptodactylidae) collected in a rice field (CA) in San Javier department, Santa Fe (Argentina), seven days after the application of chlorpyrifos and in a reference site (SR). The histological analysis revealed a significant amount (p = 0.028) and area occupied by MM (p = 0.017) in livers of CA compared to SR. Furthermore, a significant inhibition of GST activity was recorded in the CA frogs compared to the SR (p = 0.030). The histopathological and enzymatic effects provide evidences of ecotoxicological risk for anurans in rice field with CPF application.
Keywords : anurans; biomarkers; liver; rice field.