Abstract

ORTIZ-MONSALVE, Camilo; GUERRA-GONZALEZ, Jorge Mario  and  JARAMILLO-GRAJALES, Marisol. Immobilization of DNA probes on a high frequency piezoelectric biosensor. Dyna rev.fac.nac.minas [online]. 2020, vol.87, n.212, pp.163-168. ISSN 0012-7353.  https://doi.org/10.15446/dyna.v87n212.82309.

In recent years, researchers have taken to biosensors as effective tools for detection due to their portability, low-cost, fast response, and practicality. Piezoelectricity gave way to quartz crystal microbalances (QCM), of which high-frequency QCMs (HFF-QCM 100MHz) are still being researched. In this paper, we use DNA immobilization on a HFF-QCM via self-assembled monolayers (SAM) technique. Immobilization was initially verified with ATR-FTIR. Then, DNA was immobilized in real time on the HFF-QCM crystals. A variation in the phase of the signal suggests fixation of DNA to the surface, in accordance with ATR-FTIR results. A density of 629 ng/cm2 was computed. Also, a positive correlation between immobilized DNA and DNA concentration, and the appearance of a saturation point between 1 and 5 μM were shown after analysis of different DNA concentrations.

Keywords : DNA; self-assembled monolayers, SAM; biosensors; quartz crystal microbalances, QCM; high-frequency, HFF-QCM; genosensor.

        · abstract in Spanish     · text in English     · English ( pdf )