Abstract

BURGOS-PAZ, William; ROSERO-GALINDO, Carol; CARDENAS-HENAO, Heiber  and  SOLARTE-PORTILLA, Carlos. Amplified Fragments Length Polymorphisms (AFLP´s) from blood samples stored in FTA® cards for Cavia porcellus. Rev Colom Cienc Pecua [online]. 2007, vol.20, n.1, pp.67-72. ISSN 0120-0690.

A methodology that includes the use of FTA^® (Whatman Bioscience, Cambridge) to collect and store animals` blood samples and the procedures to extract and to get AFLP markers is presented in this paper. A review of the literature indicates that there are no reports concerning both aspects for the Cavia porcellus case. To reach our goal blood samples of three populations - Two native ones and other genetically improved- were obtained through heart puncture. This blood was stored in the FTA cards in order to extract, purify, amplify and analyze their DNA forms. All of the animals came from "Botana" farm of the Universidad de Nariño, located in Pasto, Colombia. For amplifying the AFLP one, three and five 1.2 mm FTA disks of approximately 75 ng of DNA per disk where used. The tests indicated that the best products to amplify and to visualize the AFLP where those ones obtained from samples of three FTA disks per animal. This suggests that 75 ng of DNA per animal is enough to generate AFLP of high quality in the Cavia porcellus` genome. We recommend the use of FTA cards to carry out genetic analyses in the Cavia porcellus, including the methodology modifications presented in this paper.

Keywords : AFLP´s; Cavia porcellus; FTA.

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