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Revista Colombiana de Ciencias Pecuarias
Print version ISSN 0120-0690
Abstract
PARRA-SUESCUN, Jaime; AGUDELO-TRUJILLO, Jorge H and LOPEZ-HERRERA, Albeiro. Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs. Rev Colom Cienc Pecua [online]. 2015, vol.28, n.1, pp.64-73. ISSN 0120-0690.
Background:Escherichia coli populations in the gut increase after weaning, releasing cell wall lipopolysaccharides (LPS), which are potent pro-inflammatory substances.Objective: this study evaluated LPS intake effects on protein expression and activity of intestinal disaccharidases and aminopetidases. Methods: sixty-four pigs (weaning age: 21 d) were fed a basal diet with added LPS (0.0, 0.3, 0.5, and 1.0 µg LPS/mg feed) during 10 days post-weaning. Pigs were slaughtered on days 1, 5, 7, and 10 post-weaning to assess small intestine molecular expression and enzyme activity. A randomized block design in a 4x4 factorial arrangement was used. Results:intestinal enzyme expression and activity was lower in pigs fed the highest LPS level (p<0.01). Enzyme values were lowest at day 10 (p<0.01). The highest gene expression and enzyme activity was observed in duodenum (p<0.01). Conclusion: data from this study suggest that LPS from E. coli decreases intestinal enzyme expression and activity.
Keywords : digestive enzymes; intestinal villi; RT-PCR; swine.