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Revista Colombiana de Ciencias Pecuarias
Print version ISSN 0120-0690On-line version ISSN 2256-2958
Abstract
OCAMPO, Pablo E et al. Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells. Rev Colom Cienc Pecua [online]. 2021, vol.34, n.3, pp.212-223. Epub Oct 10, 2022. ISSN 0120-0690. https://doi.org/10.17533/udea.rccp.v34n3a06.
Background:
Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage.
Objective:
To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs).
Methods:
MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry.
Results:
The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs.
Conclusions:
Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.
Keywords : cell differentiation; cartilage; chondrocytes; chondrogenesis; chondrogenic differentiation; collagen; corticosteroids; glucocorticoid; histology; hyaluronic acid; mesenchymal stem cell; osteoarthritis; triancinolone acetonide; viscosupplementation.