Abstract

VANEGAS CANO, Leidyjohana; MARTINEZ PERALTA, Sixta Tulia; COY BARRERA, Ericsson  and  ARDILA BARRANTES, Harold Duban. Early responses at symplastic stem level associated with the salicylic acid pathway in the interaction carnation (Dianthus caryophyllus, caryophyllaceae)- Fod (Fusarium oxysporum f. Sp. dianthi). Acta biol.Colomb. [online]. 2022, vol.27, n.2, pp.153-163.  Epub May 28, 2024. ISSN 0120-548X.  https://doi.org/10.15446/abc.v27n2.85778.

The vascular wilting caused by the Fusarium oxysporum f. sp. dianthi (Fod) is the most important disease affecting carnation crops. Understanding the nature of the interaction between the plant and the pathogen will allow the development of new alternatives for disease control. That is why on this study, we want to know the role of the salicylic acid's (SA) signaling pathway and of some enzymes associated to the plant's resistance such as phenylalanine amino lyase (PAL), polyphenol oxidase (PFO), guaiacol peroxidase (GPX) and phospholipase D (PLD), at the stem's symplastic level during the interaction with the pathogen. An in vivo assay was established using two carnation cultivars presenting contrasting levels of resistance to the disease, and the levels of SA, MeSA (Methyl salicylate) and the named enzymes were determined. Induction of the studied enzymes occurred, showing an increase at the transcriptional level in the case of the GPX enzyme. Likewise, an increase of MeSA was presented at 1 and 14 dpi, while SA was accumulated at later times. The Pearson correlation determined that on this level there is an accumulation of the MeSA hormone on 1 dpi with the levels of the enzymes GPX and PLD. We proposed that the response on the stem symplast can be activated by the signaling pathway that involves SA, affecting the secondary metabolism and the regulation of reactive species of oxygen.

Keywords : Vascular wilting; carnation; defense response; enzymatic activity; hormones.

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