Abstract

IGLESIAS, Dianella; RUIZ DE LEON, Lyselle; TORRES RODRIGUEZ, Damaris  and  COLLADO LOPEZ, Raúl. Shoot Regeneration from transformed Phaseolus vulgaris _cultivars 'ICA Pijao' and 'BAT 93' tissue. Acta biol.Colomb. [online]. 2022, vol.27, n.2, pp.240-248.  Epub May 29, 2024. ISSN 0120-548X.  https://doi.org/10.15446/abc.v27n2.86304.

The cultivation of Phaseolus vulgaris L. is widespread in the world. The genetic transformation via Agrobacterium tumefaciens, is an alternative to obtain cultivars with tolerance or resistance to adverse environmental conditions for this species. However, the cultivar-dependent condition and the low regeneration of the transformed tissue in the protocols established for P. vulgaris have limited the use of this technology to a small group of cultivars. The aim of this work was to regenerate shoots from transformed tissue in P. vulgaris cv. 'ICA Pijao' and 'BAT 93'. The effect of the concentration of the bacterial suspension, the infiltration by vacuum, the period of co-cultivation on the regeneration of shoots was determined. In addition, the effectiveness of geneticin and glufosinate-ammonium was determined during the selection stage in the regeneration of shoots from explants inoculated with A. tumefaciens. The explants V NC-1 inoculated with a bacterial suspension adjusted to an optical density of 0.1, without infiltration with vacuum and maintained for three days in co-culture presented the best regeneration response. Geneticin and glufosinate ammonium were effective in the selection of shoots. The regeneration of shoots from transformed tissue by A. tumefaciens in P. vulgaris cv. 'ICA Pijao' and 'BAT 93', not described in the scientific literature, is important for its subsequent application in breeding programs of this species.

Keywords : co-culture; explant; infiltration; selection.

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