Abstract

LOPEZ-LOPEZ, Karina et al. Molecular detection of begomoviruses isolated from weeds associated with chili pepper crops (Capsicum spp.) in Valle del Cauca, Colombia. Acta biol.Colomb. [online]. 2022, vol.27, n.3, pp.336-346.  Epub June 12, 2024. ISSN 0120-548X.  https://doi.org/10.15446/abc.v27n3.89802.

Potato yellow mosaic virus (PYMV / Co), Passionfruit leaf distortion virus (PLDV), Pepper rugose mosaic virus (PRMV), Rhynchosia golden mosaic Colombia virus (RhGMCV) are begomoviruses of agricultural interest isolated and molecularly characterized in Valle del Cauca. However, at present there is not enough information about their alternate hosts. Given that weeds play a role in viral ecology and epidemiology, this study aimed to detect the presence of these begomoviruses in weeds associated with the cultivation of chili in Valle del Cauca, Colombia. One hundred twenty-one weed plants were collected in chili-producing areas, located in seven municipalities of Valle del Cauca, which were identified at the taxonomic level. From the purified genomic DNA of these plants, the presence of the virus was evaluated by PCR using universal primers for the Begomovirus genus, and species-specific primers for PYMV/Co, PLDV, PRMV, and RhGMCV. Begomoviruses were detected in fifteen of the evaluated weed species. This is the first time that the species Ipomoea tiliacea, Melothria pendula, Caperonia palustris, Desmodium tortuosum, Desmodium intortum, Ammannia coccinea, Panicum polygonatum, Capsicum rhomboideum, Eclipta prostrata and Synedrella nodiflora have been reported as hosts of begomoviruses in Colombia. These results provide new data on the alternate hosts of begomoviruses. This information will serve to implement an integrated management plan for viral diseases, with the potential to negatively affect the yield of chili peppers and other crops in Colombia.

Keywords : coat protein; geminivirus; dicot; monocot.

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