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Revista MVZ Córdoba
Print version ISSN 0122-0268On-line version ISSN 1909-0544
Abstract
HERRERA-PEREZ, Jerónimo et al. In vitro fermentation of fibrous substrates by water buffalo ruminal cellulolytic bacteria consortia. Rev.MVZ Cordoba [online]. 2018, vol.23, n.3, pp.6860-6870. ISSN 0122-0268. https://doi.org/10.21897/rmvz.1374.
Objective.
To measure the in vitro fermentation variables of a cellulolytic bacteria consortium (CBC) isolated from a water buffalo rumen in coculture with total ruminal bacteria (TRB) on two fibrous substrates.
Materials and methods.
A CBC was isolated from the ruminal fluid of a female water buffalo in selective cellulolytic media. The experimental design was completely random with a 3x2 factorial arrangement; factors were treatments [TRB, CBC, and coculture (TRB + CBC)] and substrates (cobra grass and corn stover). Total gas and methane (CH4) production were measured at different time intervals. At 72 h, measurements were taken of pH, ammoniacal nitrogen (NH3-N), dry matter degradation (DMD), neutral detergent fiber degradation (NDFD) and total bacteria population.
Results.
Gas production with both substrates was highest (p≤0.05) in the coculture at 3, 6 and 24 h. At 48 and 72 h, gas production in the cobra grass was highest (p≤0.05) in the coculture. The coculture and TRB did not differ (p>0.05) in terms of CH4, DMD and NDFD values at 48 and 72 h. With the cobra grass, NH3-N concentration was higher (p≤0.05) in the coculture than in the TRB.
Conclusions.
The gas production and dry matter degradation values of the water buffalo rumen cellulolytic bacteria consortia indicate them to be a promising alternative for improving cobra grass structural carbohydrates degradation when in coculture with bovine ruminal bacteria.
Keywords : Ruminal bacteria; Bubalus bubalis; cellulosic coculture; degradation (Source: DeCS).