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Universitas Scientiarum
Print version ISSN 0122-7483
Abstract
GARCIA, Dabeiba Adriana; PATINO, Liliana and RUEDA, Gabriela. Cytotoxicity measurement and cytokine production in human gingival fibroblasts stimulated with Mercurius-Heel®S: Pilot Study. Univ. Sci. [online]. 2014, vol.19, n.3, pp.225-232. ISSN 0122-7483. https://doi.org/10.11144/Javeriana.SC19-3.mcpc.
Mercurius-Heel®S is a homeopathic remedy used to treat infectious diseases such as gingivitis and periodontitis, mainly in the oral cavity. The main component of this drug is the Mercurius solubilis. Its cytotoxic effect was established based on cell viability and its anti-inflammatory effect by measuring the cytokines Mercurius-Heel®S on human gingival fibroblasts (FGH). We treated cells with Mercurius-Heel®S at concentrations of 300 mg/ml to 0.0005 mg/ml and left untreated cells as a control. Cell viability was measured using the MTS® colorimetric assay and the expression of cytokines (IL1b, TNFa e IL10) present in cell supernatants was performed with Quantiquine® ELISA. From 3.7 mg/ml to 0.0005 mg/ml, we observed a significantly higher increase in cell viability in comparison to the control (lower concentrations of the drug). Cytokine production was not significantly different at different concentrations of Mercurius-Heel®S. There was no significant increase in IL1b, IL10 and TNFa and Mercurius-Heel®S showed no cytotoxic effect on FGH.
Keywords : Fibroblast; Mercurius-Heel®S; cytotoxicity; cytocines.