Abstract

HENAO URIBE, Francisco Javier; VALENCIA GIRALDO, Julián Alonso; DIAZ FRANCO, Orlando  and  RANGEL SIERRA, Marcos Yesid. EFFECTS OF ADITION OF SE MINAL PLASMA ON THE ELIMINATION OF CYTOPLASMIC DROPLETS IN OF SUS SCROFA LINAEUS, 1758 BOAR SEMEN. Bol. Cient. Mus. Hist. Nat. Univ. Caldas [online]. 2011, vol.15, n.2, pp.94-104. ISSN 0123-3068.

The cytoplasmic droplets (CDs) are remnants of the cytoplasm that are attached to the sperm after spermatogenesis. CDs constitute the most frequent sperm abnormality in pigs and are clearly related to low fertility. There are serious indications that fructose and the seminal plasma AMPc are involved in sperm maturation in the CDs detachment and in the acrosome reaction. The objective of this study was to evaluate the effect of the addition of seminal plasma in the CDs detachment in males with diagnosis of persistence of such detachment. Three boars (two with persistence of CDs and one normal) from three to five years of age, housed in the Montelindo farm at Universidad de Caldas were used in the study. These boars were performed complete seminal analysis weekly during four months. A factorial arrangement 3x3x2 (addition to the males FR with CDs persistence of 0%, 20% of SPHM and 20% of SPMCDs; 0, 60 and 120 minutes incubation, and 16, and 37ºC incubation temperature) was carried out in a randomized complete blocks design, analyzed through variance analysis and Tukey's test. Incubation of males semen with persistence of CDs with SPMCDs decreased more than 4% the CDS with regards to incubation without SP and SPHM; similarly, there was reduction of approximately 5% in CDs when increasing incubation time from 0 to 120 minutes, and about 2% when increasing incubation temperature from 16 to 37ºC.

Keywords : boar semen; cytoplasmic droplets; seminal plasma; semen quality.

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