Abstract

ESCOBAR M, Linamaría; ALFONSO R, Paola A  and  ARISTIZABAL G, Fabio A. Assessing two staining methods for cytotoxicity tests on tumor cell lines. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp.49-56. ISSN 0123-3475.

Cell staining methods are commonly used for estimating extracts or compounds’ potential cytotoxic activity when prospecting for indirect quantitative measurement of cell growth and viability. This work compared the sulphorhodamine B (SRB) staining method, which is a terminal cell density measuring assay, and the resazurin method, a cell proliferation assay which is supposed to be less toxic for the cell. The methods were compared in terms of percentage survival by evaluating MCF-7, MKN-45 and SiHa cell line sensitivity to three synthetic compounds using a 48-hour treatment period and doxorubicin HCl (a drug used in cancer treatment) as reference activity. A correlation test using the data obtained from the assays showed that there was no significant difference between the methods in the conditions used here.

Keywords : cytotoxicity; correlation; sulphorhodamine B assay; resazurin assay.

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