Abstract

BARBOSA CEPEDA, Iván Darío  and  CHAPARRO GIRALDO, Alejandro. Minimum inhibitory concentration of higromycin B in rice embryogenic calli (Oryza sativa L.). Rev. colomb. biotecnol [online]. 2011, vol.13, n.2, pp.193-198. ISSN 0123-3475.

An efficient selection system is one of the most important elements of plant genetic engineering. The purpose of this study was to evaluate the sensitivity of scutellum-derived embriogenic calli obtained from three colombian rice varieties (FEDEARROZ 2000, FEDEARROZ 50 and FEDEARROZ 369), to the selection marker hygromycin B. Aditionally, the response of these varieties to the regeneration protocol was measured. Four antibiotic concentrations were tested (25 mg/L, 50 mg/L, 75 mg/L and 100 mg/L) plus one control without hygromycin B. The results show that 50 mg/L of antibiotic in the regeneration medium is adequate for selection. This concentration prevents the formation of shoots, though the calli do not die. It was also established that the regeneration protocol is a low-efficiency system and it needs to be improved, in order to use it for colombian rice genetic engineering.

Keywords : rice Colombian varieties; FEDEARROZ 2000; FEDEARROZ 50; FEDEARROZ 369; selection system; antibiotic..

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