Abstract

BUENO, Mirian Susana; SORREQUIETA, Juan Ignacio; FELDMAN, Susana Raquel  and  A. ORTIZ, Juan Pablo. In vitro plant regeneration of Spartina argentinensis Parodi. Rev. colomb. biotecnol [online]. 2012, vol.14, n.2, pp.61-69. ISSN 0123-3475.

Spartina argentinensis Parodi is the dominant species of the temporally-flooded halophyte communities of the Santa Fe Province, Argentina. It occupies around 20,000 km² and it is mainly used as low-cost impute forage for cattle production. The objective of this work was to develop a simple method for in vitro plant regeneration of S. argentinensis that could be used for fundamental and applied research. Leaf-basal segments from both young and mature plants, roots tips and immature inflorescences were used as explants. Culture media for calli, shoot, and root induction consisted of Murashige & Skoog salts supplemented with different plant growth regulators (2,4-D; BAP or ANA). Most of the explants (with the exception of root tips) showed cell proliferation and calli formation after 30 days of culture. However, only immature inflorescences responded to shoot and root induction when calli were incubated on MS salts plus 2,4_D and BAP (0.1 and 0.01 mg.L^-1, respectively), transferred to shoot inductionmedia (MS salts plus BAP, 0.5 mg.L^-1) and then to root induction media (MS slts plus NAA 0.5 mg.L^-1). Regenerated plants were evaluated for morphological abnormalities and lignin content. Historical analysis of regenerating calli showed that shoots and roots originated via organogenesis. A low proportion of regenerated plants resulted with deficiency in chlorophyll (albino plants) and other morphological abnormalities. Interestingly, significant variations in the lignin content were detected between regenerated plants. The protocol described in this work could be used ordinarily for S. argentinensis in vitro plant regeneration and selecting somaclonal variants for breeding purposes.

Keywords : Spartina argentinensis; in vitro; organogenesis; somaclonal variation.

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