Abstract

VACCA MOLINA, Maritza; CRISTINA BONOMO, María Luisa; AVILES, Zulma  and  DIAZ, Lucía. Embriogenic calli induction and somatic proembryo formation in Pterogyne nitens Tull. Rev. colomb. biotecnol [online]. 2014, vol.16, n.2, pp.194-203. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n2.47248.

Pterogyne nitens is a tree that grows in wet forests of Northern Argentina. Due to its quality timber especies are used in afforestation, reforestation or enrichment of native forests. An alternative to conventional methods of propagation is the techniques of plant tissue using somatic embryogenic. The aim of this study is to induce the formation of embryogenic callus and of somatic obtaining proembryos, evaluating the influence of type of explant, type and concentration of growth regulators and composition of the basal medium. For induction of embryogenic callus from explants extracted cotyledonary leaves, leaflets, stems and roots of seedling of two months old are used. Three means of MSC culture, MS50 and MSMOD are also used. Each culture medium is supplemented with 2,4-D at different concentration or ANA. After 45 days the explants from cotyledon leaves in MSC media supplement with 2,4-D at concentrations of 67.87, 107.41, 113.12, 135.74 and 180.99 μM, promote greater proportion of yellow callus formation masses. With 107.41 μM 2,4-D proembryos structures formation are observed. The root segments in all treatment tested, do not develop calluses.

Keywords : Tipa colorada; micropropagation; embryogenesis; forestry; legume.

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