Services on Demand
Journal
Article
Indicators
Cited by SciELO
Access statistics
Related links
Cited by Google
Similars in SciELO
Similars in Google
Share
Revista Colombiana de Biotecnología
Print version ISSN 0123-3475
Abstract
ALBANY DE VILCHEZ, Nilca Rosa et al. Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.). Rev. colomb. biotecnol [online]. 2015, vol.17, n.1, pp.24-31. Epub July 08, 2021. ISSN 0123-3475. https://doi.org/10.15446/rev.colomb.biote.v17n1.50669.
Micropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill.) plant production. However, it has limited use due to the high production cost. Liquid media were compared to agar-gelled medium during all micropropagation stages of aloe to reduce the cost for gelling agent used. In the establishment stage, there was a higher percentage of contami nated explants in static liquid medium (25.55%) than those cultured in agar-gelled medium (11.11%), although all the explants were established independently of the culture medium used, higher height (3.81 cm) was observed in liquid medium than those growing in agar-gelled medium (3.03 cm). In the multiplication stage, explant height was higher in the recipients used for automated temporary immersion system (RITA®) (4.43 - 6.01 cm) than those cultured in agar-gelled medium (3.24 - 3.42 cm), there was no significant difference for number of shoots/explant. All shoots had roots at 30 days independently of used culture media (static liquid or agar-gelled media). Shoot height, number and root length had similar values in both culture media. The implementation of liquid media and automated temporary Immersion system RITA® may allow to reduce production costs of gelling agent used, it represents an approach for the commercial micropropagation of aloe.
Keywords : Tissue culture; gelling agents; RITA®; temporary immersion system.