Abstract

CARBONELL, Xiomara Ruth; VERDES, Patricia Estela  and  LEPORATI, Jorge Leandro. Preliminary evaluation of in vitro callogenesis and organogenesis Leptochloa crinita (Lag.) P. M. Peterson & N. Snow. Rev. colomb. biotecnol [online]. 2022, vol.24, n.1, pp.19-26.  Epub Sep 01, 2022. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v24n1.99374.

In San Luis (Argentina), land-use change due to a variety of socioeconomic, climatic, technological and cultural factors has caused the loss of biological diversity, fragmentation and habitat destruction, compromising the existence of native flora, such as in the case of Leptochloa crinita (Lag.) PM Peterson & N. Snow, a species with good forage aptitude from the Chaco forest. In this context, the present work aimed to apply biotechniques as an alternative for an appropriate propagation of this gene pool. In vitro organogenesis and callogenesis of several explants were evaluated with different growth regulators on a Murashige and Skoog nutrient medium. Treatment with 6 mg l-1 of 2,4-D stimulated callogenesis, while combined auxin and cytokinin treatments presented the highest rates of morphogenesis. The development of this biotechnique makes it possible to have adequate methodologies to initiate in vitro conservation trials, analysis of genetic variability and the initiation of domestication and breeding programs.

Keywords : forage plants; native plants; growth regulators; morphogenesis.

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