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Infectio
Print version ISSN 0123-9392
Abstract
SANCHEZ, Magda and JOSA M, Diego. Rapid detection of carbapenemase-producing Enterobacteriaceae in rectal swabs of colonized neonatal patients. Infect. [online]. 2021, vol.25, n.2, pp.89-92. Epub May 10, 2021. ISSN 0123-9392. https://doi.org/10.22354/in.v25i2.925.
Objective:
To detect the presence of carbapenemase-producing Enterobacteriaceae in rectal swabs of neonates by means of laser nephelometry technique and characterization of the type of carbapenemase by immunochromatographic test.
Materials and Methods:
Descriptive cross-sectional study. 57 neonatal patients were included; They underwent rectal screening upon admission to the ICU, using swabs which were processed by HB&L Carbapenemase laser nephelometry (Alifax®) and characterization of the type of carbapenemase by RESIST-3 rapid immu nochromatography (Coris BioConcept®).
Results:
We found a high percentage of rectal colonization (22.9%) corresponding to 13 positive swabs and 44 samples (77.1%) were negative by laser nephelome try. Identification of carbapenem-resistant Klebsiella pneumoniae was obtained by VITEK 2® in the 13 isolates and the immunochromatographic test revealed the presence of blaKPC carbapenemases in these isolates.
Discussion:
Studies show increased colonization by carbapenemase-producing microorganisms in neonates. The results of this study demonstrate that a significant percentage of neonates who enter Neonatal Care Units are colonized with Enterobacteriaceae that produce carbapenemases in the intestinal tract. This constitu tes a potential risk for its spread and subsequent development of outbreaks, where the importance of implementing active surveillance strategies such as rectal screening for the timely detection of colonized neonates arises.
Keywords : Carbapenemases; rectal colonization; neonate; nephelometry; immunochromatographic assay; Enterobacteria.