Abstract

ISAZA, Carolina; ESCOBAR, Martha Isabel; MONTOYA, Antonio  and  SANCHEZ, Jairo . Chromosomal alterations of meningioma cells and its relation to growth in vitro. Colomb. Med. [online]. 2006, vol.37, n.1, pp.61-66. ISSN 1657-9534.

Objectives: To demonstrate the frequency of chromosomic alterations and their relationship to the growth velocity of in vitro meningioma cells cultures. Data obtained in previous studies have shown that there is a kinship between chromosomic alterations, the risk of recurrence and tumor stage. Materials and methods: Samples taken during meningioma resection surgery from any location were cultured. The samples were obtained from patients at the University Hospital, the Rafael Uribe Clinic and private clinics, previous informed consent either from patients or their relatives. Cell cultures were performed, speed of growth was measured, and the karyotype was obtained in order to define the chromosomic alterations present in each tumor. Results: 47% of the meningiomas studied showed different chromosomic alterations or besides monosomy of the chromosome 22 that according to previous publications are associated to tumoral progression and represent a recurrence risk greater than 10%. Conclusions: The cytogenetic studies and the in vitro cellular growth velocity of the tumoral cells could be excellent complements of the surgical result and the pathology study, contributing to establish the risk of recurrence, so helping to define the patient’s prognosis.

Keywords : Meningioma; Chromosomes; In vitro tumoral cell culture.

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