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Revista Ciencias de la Salud
Print version ISSN 1692-7273On-line version ISSN 2145-4507
Abstract
AMORTEGUI, Ángela Ximena and RAMIREZ, Sandra Rocío. Primary culture of human keratinocytes planted on pigs intestine Submucosa. Rev. Cienc. Salud [online]. 2008, vol.6, n.3, pp.08-22. ISSN 1692-7273.
Tissue engineering, in the fields of skin regeneration, seeks to overcome the limitations associated with the use of immediate auto-grafts, since choosing the donor region of the patient constitutes a risk for the patient himself and is insufficient if the injury that has to be repaired is very large. The use of the pigs small intestine submucosa (SIS) has being proved as a filling biomaterial to treat injuries, because of its special composition, it lowers pain and inflammation since its first application and it favours early mobility to the wounded area. The present study developed a protocol for the primary culture of human keratinocytes from infant foreskins, and used small intestinal submucosa (SIS) like culture substrate. Cellular adherence potential and proliferation capability of the keratinocytes over this substrate was evaluated.
Keywords : tissue engineering; tissue culture techniques; guided tissue regeneration; kerationocytes; SIS.