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Revista Colombiana de Biotecnología
versión impresa ISSN 0123-3475
Resumen
SALCEDO MENDOZA, Jairo G; GALAN LOPEZ, Jorge E y PARDO FLOREZ, Luz Marina. Hydrolysis Enzymatic of crop residues sugar cane. Rev. colomb. biotecnol [online]. 2012, vol.14, n.1, pp.171-181. ISSN 0123-3475.
In this research, a delignified substrate from crops residues sugar cane residues (leaves and top cane) was hydrolyzed using an enzyme preparation with 27.53 FPU. This enzyme was obtained from trade. Hydrolysis was carried out to pH of 4.2 and a temperature of 50 oC. Models of inhibition models substrate, glucose and total inhibition product was analyzed. The results showed that models that best fit the data experimental was the models competitive glucose inhibition (Km= 20.37, Vmax=39 and ki= 0.442). In the event that E/S is above 0.5, can applied kinetic models of Michaelis - Menten.
Palabras clave : Inhibition; kinetic models; leaves and tops cane; enzyme cocktail.