Resumen

HERNANDEZ-GUZMAN, Anngie Katherine  y  GUZMAN- BARNEY, M. Mónica. Potato yellow vein virus detection in different organs of Solanum tuberosum Phureja group cv Criolla Colombia by conventional and real time qRT-PCR. Rev. colomb. biotecnol [online]. 2014, vol.16, n.1, pp.74-85. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n1.44226.

Potato yield in Colombia could be affected by the infection with different viral pathogens, among which, Potato vein yellow virus (PYVV) could reduce potato production by 30% to 50%. PYVV has been diagnosed molecularly in symptomatic and symptomless leaves samples by conventional RT-PCR. However, the PYVV detection and distribution in different organs of symptomatic and symptomless plants have not been reported until now. The aim of this research was to detect and analyze PYVV distribution in different organs of infected S. tuberosum group Phureja cv. Criolla Colombia (papa criolla) plants using conventional and real time qRT-PCR usindTaqMan® probes. It was achieved to detect the virus in all analyzed organs (leaflets, petiole, peduncle, anther, petals, aerial and underground stem) by both techniques; however, qRT-PCR was 100 times more sensitive than the conventional technique. Additionally, the absolute quantification of coat major protein gene (CP) was determined. The results shown that in non symptomatic plants (NS), PYVV was distributed homogenously with an average CP gene copy number of 4.09 × 10⁷ ± 2.35 × 10⁷, while in symptomatic moderate and severe plants (M) or (S) the viral load was greater (6.82×10⁸±1.74×10⁸) with an heterogeneous distribution regarding the organ and with greater accumulation in the aerial organs. The results presented in this study will be important for PYVV detection and further studies on the virus biology, host and vector relations. The information should be useful to farmers, breeders, indexing and seed certification programs.

Palabras clave : TaqMan® probes; PYVV; distribution; Crinivirus; absolute quantification.

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