Resumen

MORENO CONN, Lina M.; PEREZ, Adrián; RAMIREZ, Margarita  y  FRANCO, Marcela. Effect of storage temperature on the viability of symbiotic nitrogen fixing bacteria used in the preparation of biological inoculants for crop pea (Pisum sativum) and soybean (Glycine max). Rev. colomb. biotecnol [online]. 2014, vol.16, n.2, pp.45-56. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n2.47244.

Nitrogen is an element required in large amounts by most crops, however, in soil is one of the most limiting and located in ways not available to the plant and must be incorporated through chemical fertilization or by biological fixation conducted by microorganisms. Fixing symbiotic bacteria nitrogen has been widely used for the production of inoculants, becoming a viable alternative to improve crop yields through a better supply of this element. These inoculants to be marketed must meet certain requirements quality to ensure the smooth operation of the product. This study assessed the viability of the strains ICA L9 and ICA J96 inoculants stored at temperatures 4±2, 18±3 y 28±2°C for 180 days and the biological activity in pea and soybean plants. The strains used belong to the collection of work Germplasm Bank CORPOICA Microorganisms; for biological assays were used pea seeds variety "Santa Isabel" and soybean variety "Superior Corpoica 6". In assessing the viability of inoculants stored at 4±2°C, 18±3°C y 28±2°C, evidenced that the temperature did not affect the survival and the concentration of rhizobia after 180 days of storage allowing to observe a number of colony forming units per gram than 10s, value that guarantees the quality of the inoculant. Concerning the biological activity, it was observed that the strains were inoculated infective and effective biological nitrogen fixation, absolute compared to controls.

Palabras clave : Nitrogen; inoculants; Germplasm Bank; pea; soybean.

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