Resumen

LIZ, Castro; ELVIO, Gayozo  y  NATALIA, Méndez. Evaluation of the utility of microsatellite markers in the poultry population rustipollos. Rev. colomb. biotecnol [online]. 2021, vol.23, n.2, pp.41-46.  Epub 18-Feb-2022. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v23n2.94961.

Molecular markers are a very useful tool for genetic diversity studies, allowing the identification of populations with particular genetic characteristics, in order to establish conservation and genetic improvement programs. The objective of this study was to evaluate the degree of information generated by a panel of 30 microsatellite markers in the Rustipollos poultry population. Blood samples were obtained from 50 individuals, the fragments were amplified by PCR, using 30 microsatellites recommended by FAO-ISAG for biodiversity studies in chickens. The estimation of the fragment sizes was carried out in an ABI Prism 377 automatic sequencer. The number of alleles per locus and the Polymorphic Information Content (PIC) were determined using the Microsatellite-Toolkit program. The total number of alleles reported was 99 in the 30 microsatellite markers, with a mean value of 3.3 ± 1.06 alleles per locus. The PIC determination registered an average of 0.46, with a range of 0.18 to 0.76 in the MCW016 and ADL278 markers, respectively. 43% of the markers used were highly informative for the population evaluated. In general, microsatellite markers proved to be useful for genetic studies in the Rustipollos poultry population.

Palabras clave : Polymorphism information content; conservation; chickens; Paraguay; genetic variation.

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